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KMID : 0380020130280010042
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 1 p.42 ~ p.47
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Optimization of Cellulolytic Enzyme Production for newly isolated Bacillus sp. H9-1 from Herbivore Feces
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Yoon Young-Mi
An Gi-Hong
Kim Jung-Kon
Cha Young-Lok
Park Yu-Ri
Ahn Jong-Woong
Moon Youn-Ho
Ahn Seung-Hyun
Koo Bon-Cheol
Park Kwang-Geun
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Abstract
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This study was performed to find cellulolytic strain of enzymatic saccharification for bioethanol production. Cellulolytic strains were isolated from 59 different feces of herbivores from Seoul Grand Park located in Gwacheon Gyeonggi-Do. The celluloytic strain was selected by congo red staining and DNS method. Among the isolated strains, H9-1 strain isolated from the feces of rabbit has the highest CMCase activity. H9-1 strain was identified as Bacillus sp. based on 16S rDNA gene sequencing. The optimal conditions for CMCase activity by Bacillus sp. H9-1 were at 40oC and at initial pH 8.
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KEYWORD
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bioethanol, herbivore feces, Bacillus sp. H9-1, CMCase activity
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